Commit 686f8d2f by Ashish Sawhney

project

parent 7df477c7
Pipeline #89585 passed with stages
in 21 seconds
# 1. Principles and practices
# 1. Principles and Practices
This week I worked on defining my final project idea and started to getting used to the documentation process.
## a very brief and general introduction to mass spectrometers
## Background
Mass spectrometers come in various flavors. Layout of a typical mass spectrometer is shown in Figure 1.1 below. Its major components include:
a very brief and general introduction to mass spectrometers
Mass spectrometers come in various flavors. Layout of a typical mass spectrometer is shown in Figure 1.1 below. Its major components include:
a. a sample inlet: for introducing the sample into the mass spectrometer, can be direct injection, or eluate of a gas or liquid chromatograph;
b. an ion source: for generating gas-phase ions of analytes in the sample;
c. an m/z analyzer: for separating analyte ions (formed in the previous step) on the basis of their m/z values;
d. a detector: for generating a signal for the ions that hit it, and recording their m/z values and abundances (as intensities); and
e. a data acquisition and analysis system: for operating the mass spectrometer to acquire data; and for post-processing of raw data
a. a sample inlet: for introducing the sample into the mass spectrometer, can be direct injection, or eluate of a gas or liquid chromatograph;
b. an ion source: for generating gas-phase ions of analytes in the sample;
c. an m/z analyzer: for separating analyte ions (formed in the previous step) on the basis of their m/z values;
d. a detector: for generating a signal for the ions that hit it, and recording their m/z values and abundances (as intensities); and
e. a data acquisition and analysis system: for operating the mass spectrometer to acquire data; and for post-processing of raw data
![](../images/week01/LayoutMassSpectrometer.jpg)
##### Figure 1.1: Layout of a typical mass spectrometer showing its major components: a sample inlet; an ion source; an m/z analyzer; a detector; and a data acquisition and analysis system.
LayoutMassSpectrometer
Figure 1.1: Layout of a typical mass spectrometer showing its major components: a sample inlet; an ion source; an m/z analyzer; a detector; and a data acquisition and analysis system.
There are different options (or designs) available for all parts of a mass spectrometer, but not every design of each part is compatible with every design of the other parts, and so mass spectrometers of different configurations are available from various instrument manufacturers. When buying a mass spectrometer, the applications (or purposes) for which it is needed is a major criterion influencing the choice of configuration.
There are different options (or designs) available for all parts of a mass spectrometer, but not every design of each part is compatible with every design of the other parts, and so mass spectrometers of different configurations are available from various instrument manufacturers. When buying a mass spectrometer, the applications (or purposes) for which it is needed is a major criterion influencing the choice of configuration.
I would like to build a low cost chromatography/mass spectrometry system as proof of concept, and as an exercise to learn different skills that go into instrumentation building. Cost, both initial and operational, is a major criterion influencing buying decision when considering such instrumentation. A simple liquid chromatography-electrospray ionization-triple quadrupole mass spectrometer (LC-ESI-QqQ MS) can easily cost USD 150,000 - USD 200,000. For a lot of laboratories, and purposes, such initial costs can be prohibitively expensive, and so, I am particularly interested in building a low cost electrospray ionization-(single) quadrupole mass spectrometer (ESI-Q MS) (Figure 1.2).
## Research
"Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum."
> "Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum."
## Useful links
- [Jekyll](http://jekyll.org)
- [Google](http://google.com)
- [Markdown](https://en.wikipedia.org/wiki/Markdown)
## Code Example
Use the three backticks to separate code.
```
// the setup function runs once when you press reset or power the board
void setup() {
// initialize digital pin LED_BUILTIN as an output.
pinMode(LED_BUILTIN, OUTPUT);
}
// the loop function runs over and over again forever
void loop() {
digitalWrite(LED_BUILTIN, HIGH); // turn the LED on (HIGH is the voltage level)
delay(1000); // wait for a second
digitalWrite(LED_BUILTIN, LOW); // turn the LED off by making the voltage LOW
delay(1000); // wait for a second
}
```
## Gallery
![](../images/sample-photo.jpg)
## Video
### From Vimeo
<iframe src="https://player.vimeo.com/video/10048961" width="640" height="480" frameborder="0" webkitallowfullscreen mozallowfullscreen allowfullscreen></iframe>
<p><a href="https://vimeo.com/10048961">Sound Waves</a> from <a href="https://vimeo.com/radarboy">George Gally (Radarboy)</a> on <a href="https://vimeo.com">Vimeo</a>.</p>
### From Youtube
<iframe width="560" height="315" src="https://www.youtube.com/embed/jjNgJFemlC4" frameborder="0" allow="autoplay; encrypted-media" allowfullscreen></iframe>
## 3D Models
<div class="sketchfab-embed-wrapper"><iframe width="640" height="480" src="https://sketchfab.com/models/658c8f8a2f3042c3ad7bdedd83f1c915/embed" frameborder="0" allow="autoplay; fullscreen; vr" mozallowfullscreen="true" webkitallowfullscreen="true"></iframe>
<p style="font-size: 13px; font-weight: normal; margin: 5px; color: #4A4A4A;">
<a href="https://sketchfab.com/models/658c8f8a2f3042c3ad7bdedd83f1c915?utm_medium=embed&utm_source=website&utm_campaign=share-popup" target="_blank" style="font-weight: bold; color: #1CAAD9;">Dita&#39;s Gown</a>
by <a href="https://sketchfab.com/francisbitontistudio?utm_medium=embed&utm_source=website&utm_campaign=share-popup" target="_blank" style="font-weight: bold; color: #1CAAD9;">Francis Bitonti Studio</a>
on <a href="https://sketchfab.com?utm_medium=embed&utm_source=website&utm_campaign=share-popup" target="_blank" style="font-weight: bold; color: #1CAAD9;">Sketchfab</a>
</p>
</div>
![](../images/week01/BlockDgmESISingleQuad.jpg)
##### Figure 1.2: Block diagram of an electrospray ionization-(single) quadrupole mass spectrometer (ESI-Q MS).
\ No newline at end of file
Markdown is supported
0% or
You are about to add 0 people to the discussion. Proceed with caution.
Finish editing this message first!
Please register or to comment